| 摘要: |
| 目的 探究在页岩气生产过程中的关键腐蚀细菌,解析其中细菌潜在的演替规律。方法 利用16S rRNA高通量测序分析技术对威远地区页岩气某平台配液用水和采出水菌群进行检测。结果 清水配液的细菌结构与采出水、回用返排液完全不同,压裂后细菌的丰富度及多样性降低；关键腐蚀细菌包括弓形杆菌属(Arcobacter)、海细菌属(Marinobacterium)、假单胞菌属(Pseudomonas)等；清水配液中细菌群落是由嗜温、好氧、硫酸盐还原和产酸等功能较弱的细菌为主,而回用返排液中存在大量具有硫代谢、产酸和产生物膜功能的细菌,经压裂后,采出水中以兼性厌氧、厌氧或严格厌氧,即具有硫酸盐还原、硫还原、硫氧化、产酸、产生物膜、铁还原等功能的细菌为主,并鉴定出部分嗜热细菌。结论 可为进一步研究集输管道细菌腐蚀及管道防护提供理论与技术支持。 |
| 关键词: 页岩气 配液用水 采出水 高通量测序 细菌多样性 腐蚀细菌 |
| DOI：10.3969/j.issn.1007-3426.2023.05.014 |
| 分类号: |
| 基金项目:川庆钻探公司-西南石油大学工程技术联合研究院科技计划项目“威远页岩气微生物腐蚀深入研究及防治措施优化评价”(CQXN-2021-10) |
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| Succession law of bacterial community in fracturing fluid preparation water and produced water of a platform in Weiyuan shale gas block |
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Zhang Qing1, Jing Yudi2, Chen Zhiyi2, Yue Ming1, Bu Chengzhong1, Zhang Teng1, Tan Peng3, Sun Yu4, Xu Bo2
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1. Shale Gas Exploration and Development Department, CNPC Chuanqing Drilling Engineering Co., Ltd., Chengdu, Sichuan, China;2. College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, Sichuan, China;3. Supervision Center, PetroChina Tarim Oilfield Company, Korla, Xinjiang, China;4. Safety and Environmental Protection Quality Supervision & Testing Research Institute, CNPC Chuanqing Drilling Engineering Co., Ltd., Deyang, Sichuan, China
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| Abstract: |
| Objective The study is aiming to explore the key corrosion bacteria in the production process of shale gas, and analyze the potential succession law of bacteria. Methods The 16S rRNA high-throughput sequencing analysis technology was used to sequence the bacterial flora in the fracturing fluid preparation water and produced water of a shale gas platform in Weiyuan area. Results The bacterial structure of fresh water is completely different from that of produced water and recycled flowback fluid, the richness and diversity of bacteria decreased after fracturing. The key corrosive bacteria include Arcobacter, Marinobacterium and Pseudomonas, etc. The bacterial community in the freash water is mainly composed of mesophilic, aerobic bacteria with weak functions such as sulfate reduction and acid production. While in the recycled flowback fluid, there are a large number of bacteria with functions of sulfur metabolism, acid production and biofilm production. After fracturing, in the produced water there are mainly facultative anaerobic, anaerobic or strictly anaerobic bacteria with functions such as sulfate reduction, sulfur reduction, sulfur oxidation, acid production, biofilm production, iron reduction, and some thermophilic bacteria were identified. Conclusion This provides theoretical and technical support for further research on bacterial corrosion of gathering and transportation pipelines and pipeline protection. |
| Key words: shale gas fracturing fluid preparation water produced water high-throughput sequencing bacterial diversity corrosion bacteria |
