高效液相色谱法测定有机硫化合物浓度

Determination of the content of organic sulfide compounds by HPLC

关键词:  高效液相色谱  有机硫化合物  油品 

Key words:  high performance liquid chromatography (HPLC)  organic sulfide compound  diesel oil

摘要:

建立了高效液相色谱同时测定苯硫醚、苯并噻吩、二苯并噻吩和4,6-二甲基二苯并噻吩的方法。在正十六烷为溶剂、90%(质量分数)甲醇水溶液为流动相、流速为1 mL/min、检测波长为254 nm的条件下,4种组分在10 min内得到良好分离。线性质量浓度范围分别为3.1~350 μg/mL、2.3~400 μg/mL、3.4~500 μg/mL和5.0~300 μg/mL,检测限分别为1.98 μg/mL、1.59 μg/mL、1.47 μg/mL和2.05 μg/mL；并有较好的精密度,稳定性和回收率。实验证明,该法操作简便、分析快速、结果准确,可用于不同来源及不同加工工艺的石油产品中有机硫化合物的同时检测。 

Abstract:

A method for simultaneous determination of diphenyl sulfide,benzothiopene,dibenzothiopene and 4,6-dimethyl dibenzothiophene by high performance liquid chromatography (HPLC) was proposed. While the n-hexadecane was solvent, the mobile phase was aqueous solution of 90 wt% methanol and flow rate of 1 mL/min, the detection wavelength was in 254 nm, the results showed that four components could be well separated in 10 minutes. Linearity of four analyses were found in range of 3.1~350 μg/mL, 2.3~400 μg/mL, 3.4~500 μg/mL and 5.0~300 μg/mL, and the detection limit were 1.98 μg/mL, 1.59 μg/mL, 1.47 μg/mL and 2.05 μg/mL respectively, which had good precision, stability and recovery. The experimental results showed that the method was simple,rapid and accurate, and could be used for determination of organic sulfide compound in petroleum products from different source and processing technology.